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KMID : 0624620170500050247
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BMB Reports
2017 Volume.50 No. 5 p.247 ~ p.256
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CRISPR system for genome engineering: the application for autophagy study
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Cui Jianzhou
Chew Shirley Jia Li
Shi Yin
Gong Zhiyuan
Shen Han-Ming
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Abstract
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CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research.
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KEYWORD
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Autophagy, CRISPR/Cas9, gRNA, Off-target, PAM
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